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Protocols

Equipment and Software

Nikon Camera Control
Freeze-Dryer Protocol

MIMS Procedures

MIMS Procedures

Quantitative Image Analysis Procedures

Quantitative Image Analysis Procedures
Example of Region of Interest Analysis

Sample Preparation

Fixing, Staining, Embedding, Trimming, and Microtomy

Embedding Media
Embedding Method
Formaldehyde-Glutaraldehyde-Picric Acid (FGP) Fixative
Osmium Tetroxide Fixative
Toluidine Blue Stain
Uranyl Acetate Stain in Maleate Buffer

Sample Holders and Silicon Chips

Washing and Sterilizing Silicon Chips
Washing Sample Holders, Vehicles, and Screws
*Please note: These protocols are works in progress. Users are strongly encouraged to contact the NRIMS staff prior to using one of these protocols to verify that it is current.

We encourage you contact us with any questions, feedback, comments, or corrections.

Updated: May 01 2008

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